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KMID : 1132720100080010050
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Genomics & Informatics
2010 Volume.8 No. 1 p.50 ~ p.57
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Effects of Mercuric Chloride on Gene Expression in NRK-52E Cells
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Ahn Joon-Ik
Baik Si-Yeon
Ko Moon-Jeong
Shin Hee-Jung
Chung Hye-Joo
Jeong Ho-Sang
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Abstract
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Mercuric chloride, a model nephrotoxicant was used to elucidate time- and dose- dependent global gene expression
changes associated with proximal tubular toxicity. Rat kidney cell lines NRK-52E cells were exposed for 2, 6 and 12 hours and with 3 different doses of mercuric chloride. Cell viability assay showed that mercuric chloride had toxic effects on NRK-52E cells causing 20% cell death (IC20) at 40μM concentration. We set this IC20 as high dose concentration and 1/5
and 1/25 concentration of LC20 were used as mid and low concentration, respectively. Analyses of microarray data revealed that 738 genes were differentially expressed (more than two-fold change and p＜0.05) by low concentration of mercuric chloride at least one time point in NRK-52E cells. 317 and 2,499 genes were differentially expressed at mid and high concentration of mercuric chloride, respectively. These deregulated genes showed a primary involvement with protein trafficking (CAV2, CANX, CORO1B), detoxification (GSTs) and immunity and defense (HMOX1, NQO1). Several of these
genes were previously reported to be up-regulated in proximal tubule cells treated with nephrotoxicants and might be aid in promoting the predictive biomarkers for nephrotoxicity.
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KEYWORD
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gene expression, mercuric chloride, nephrotoxicity, NRK-52E cells
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